Single-Molecular Imaging of Rotaxane Based on Glass Substrates : Observations of Rotary Movement of a Rotor
We observed a single rotaxane molecule based on a-cyclodextrin (a-CD) attached to a fluorescence probe on glass substrates and estimate the rotary movement of a-CD as a rotor, which was measured by total internal reflection fluorescence microscopy (TIRFM). When a single fluorescence molecule was observed by TIRFM, the luminescence spot measured about 150 nm. Even if a-CD with a chromophore could show a rotary movement centering around an axis molecule of a rotaxane on a glass substrate, the three dimensional rotary movement of a-CD is difficult to be directly observed by conventional fluorescence microscopy techniques . Hence, we have employed the defocused wide-field imaging technique, which is manipulated by defocusing on a chromophore about 1 mm. The emission transition dipole orientation of a single chromophore molecule is determined by the characteristic emission intensity distribution. Herein, we observe the rotary movement of a-CD on rotaxane by measuring the emission dipole orientation of the chromophores.
Nishimura, D.; Takashima, Y.; Aoki, H.; Takahashi, T.; Yamaguchi, H.; Ito, S.; Harada, A.
"Single-Molecular Imaging of Rotaxane Based on Glass Substrates :
Observations of Rotary Movement of a Rotor"
Angew. Chem. Int. Ed. 2008, 47(32), 6077-6079.
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