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Supramolecular Assembly of Bio-Related Polymers and Their Functionalization |
Novel Biosensing Method
Linear Antibody Supramolecules: Application for a Novel Biosensing Method
In this study, methyl viologen (MV) is selected as one of the target molecules to be detected. Although viologens are harmful, they are well-known functional molecules which act as herbicides and electron acceptors. MV has been suggested as a potential etiologic factor in Parkinson’s disease because of the structural similarity to the known dopaminergic neurotoxicant, 1-methyl-4-phenyl-1,2,3,6- tetrahydropyridine. Anti-viologen antibodies may be expected to be useful not only as a highly sensitive reagent for viologens but also as a functional material to control the electron transfer from electron donors to viologens. However, the detection of viologens at low concentrations is difficult owing to the low sensitivity (small response) in a common SPR biosensor technique using corresponding antibodies. To improve the sensitivity, it is important to detect MV as a large response signal caused by the antibody bindings. A solution to this problem is thought to be the inhibition of the supramolecular formation between the anti-viologen antibody and viologen dimer by MV. A small amount of MV can be detected as a decrease in signal enhancement due to the inhibition of the complex formation between viologen dimer and antibody by MV, compared with the signal intensity of complete supramolecular formation between the antibody and viologen dimer. The sensitivity in this system was 140-fold larger than that in the simple addition of MV to the antibody immobilized to the surface of the sensor chip.
- Supramolecular Formation of Antibodies with Viologen Dimers: Utilization for Amplification of Methyl Viologen Detection Signals in Surface Plasmon Resonance Sensor. Yamaguchi, H.; Harada A. Biomacromolecules, 2002, 3, 1163 - 1169.
- Method for detecting antigen or antibody with high sensitivity. Harada, Akira; Yamaguchi, Hiroyasu. (Foundation for Scientific Technology Promotion, Japan). Patent (Jpn. Kokai Tokkyo Koho, 2002).