発表論文など
2025
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A β-cap on the FliPQR protein-export channel acts as the cap for initial flagellar rod assembly.
Kinoshita M, Miyata T, Makino F, Imada K, Namba K, Minamino T.
Proc Natl Acad Sci U S A.122(34):e2507221122 (2025) -
Cryo-EM Structure of the Flagellar Motor Complex from Paenibacillus sp. TCA20.
Onoe S, Nishikino T, Kinoshita M, Takekawa N, Minamino T, Imada K, Namba K, Kishikawa JI, Kato T.
Biomolecules.15(3):435 (2025) -
Assembly Formation of P65 Protein, Featured by an Intrinsically Disordered Region Involved in Gliding Machinery of Mycoplasma pneumoniae.
Yabe M, Toyonaga T, Kinoshita M, Furukawa Y, Hamaguchi T, Tahara YO, Arai M, Imada K, Miyata M.
Biomolecules.15(3):429 (2025) -
Sodium-Dependent Conformational Change in Flagellar Stator Protein MotS from Bacillus subtilis.
Takekawa N, Yamaguchi A, Nishiuchi K, Uehori M, Kinoshita M, Minamino T, Imada K.
Biomolecules.15(2):302 (2025) -
Structural basis for assembly and function of the Salmonella flagellar MS-ring with three different symmetries.
Kinoshita M, Makino F, Miyata T, Imada K, Namba K, Minamino T.
Commun Biol.8(1):61 (2025) - V 型線毛蛋白質の結晶化と予備的結晶学研究.
小島嶺, 庄子幹郎, 柴田敏史, 竹川宜宏, 今田勝巳.
Spring-8/SACLA利用研究成果集. 13(1) 29-31 (2025) - Key structural differences between green and red fluorescent proteins.
Imamura H, Imada K.
SPring8 Research Frontiers 2024, 22-23 (2025).
Original Papers
Reviews, Proceedings and Books
2024
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Structural insight into sodium ion pathway in the bacterial flagellar stator from marine Vibrio.
Nishikino T, Takekawa N, Kishikawa JI, Hirose M, Kojima S, Homma M, Kato T, Imada K.
Proc Natl Acad Sci U S A.122(1):e2415713122 (2024) -
Structural analysis of S-ring composed of FliFG fusion proteins in marine Vibrio polar flagellar motor.
Takekawa N, Nishikino T, Kishikawa J-i, Hirose M, Kinoshita M, Kojima S, Minamino T, Uchihashi T, Kato T, Imada K, Homma M.
mBio.15(10):e0126124 (2024) - 緑色蛍光タンパク質を赤色に ―赤色化の構造基盤―.
今田勝巳, 今村博臣.
日本結晶学会誌. 66(4) 217-218 (2024) - 目で見るバイオ：緑色蛍光タンパク質から赤色蛍光タンパク質を創り出す.
今村博臣, 今田勝巳.
バイオサイエンスとインダストリー. 82(3) 280-281 (2024) - 緑色蛍光蛋白質の改変による 新規赤色蛍光蛋白質の開発.
今村博臣, 今田勝巳.
臨床免疫・アレルギー科. 81(5) 504-510 (2024)
Original Papers
Reviews, Proceedings and Books
2023
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Red fluorescent proteins engineered from green fluorescent proteins.
Imamura H, Otsubo S, Nishida M, Takekawa N, Imada K.
Proc Natl Acad Sci U S A.120(45):e2307687120 (2023) -
In Vitro Flagellar Type III Protein Transport Assay Using Inverted Membrane Vesicles.
Imada K, Terashima H.
Methods Mol Biol.2646:17-26 (2023)
Original Papers
Reviews, Proceedings and Books
2022
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ATP-dependent polymerization dynamics of bacterial actin proteins involved in Spiroplasma swimming.
Takahashi D, Fujiwara I, Sasajima Y, Narita A, Imada K, Miyata M.
Open Biol.12(10):220083 (2022) -
Structure of MotA, a flagellar stator protein, from hyperthermophile.
Nishikino T, Takekawa N, Tran DP, Kishikawa JI, Hirose M, Onoe S, Kojima S, Homma M, Kitao A, Kato T, Imada K.
Biochem Biophys Res Commun.631:78-85(2022) -
Structural basis of ubiquitin recognition by a bacterial ovarian tumor deubiquitinase LotA.
Takekawa N, Kubori T, Iwai T, Nagai H, Imada K.
J Bacteriol.204(1):e0037621(2022)
Original Papers
2021
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Structure of the bacterial flagellar hook cap provides insights into a hook assembly mechanism.
Matsunami H, Yoon YH, Imada K, Namba K, Samatey FA.
Commun Biol.4(1):1291(2021) -
ZomB is essential for chemotaxis of Vibrio alginolyticus by the rotational direction control of the polar flagellar motor.
Takekawa N, Nishikino T, Hori K, Kojima S, Imada K, Homma M.
Genes Cells.26(11):927-937(2021) -
Native flagellar MS ring is formed by 34 subunits with 23-fold and 11-fold subsymmetries.
Kawamoto A, Miyata T, Makino F, Kinoshita M, Minamino T, Imada K, Kato T, Namba K.
Nat Commun.12(1):4223(2021) -
A slight bending of an α-helix in FliM creates a counterclockwise-locked structure of the flagellar motor in Vibrio
Takekawa N, Nishikino T, Yamashita T, Hori K, Onoue Y, Ihara K, Kojima S, Homma M, Imada K.
J Biochem.170(4):531-538(2021) -
The FlhA linker mediates flagellar protein export switching during flagellar assembly.
Inoue Y, Kinoshita M, Kida M, Takekawa N, Namba K, Imada K, Minamino T.
Commun Biol.4(1):646(2021) -
A new L-arginine oxidase engineered from L-glutamate oxidase.
Yano Y, Matsuo S, Ito N, Tamura T, Kusakabe H, Inagaki K, Imada K.
Protein Sci.30(5):1044-1055(2021) - Two distinct conformations in 34 FliF subunits generate three different symmetries within the flagellar MS-Ring.
Takekawa N, Kawamoto A, Sakuma M, Kato T, Kojima S, Kinoshita M, Minamino T, Namba K, Homma M, Imada K.
mBio.12(2):e03199-20(2021) - Structural basis of enzyme activity regulation by the propeptide of l-lysine α-oxidase precursor from Trichoderma viride.
Kitagawa M, Ito N, Matsumoto Y, Saito M, Tamura T, Kusakabe H, Inagaki K, Imada K.
J Struct Biol X.5:100044(2021)
Original Papers
2020
- PorA, a conserved C-terminal domain-containing protein, impacts the PorXY-SigP signaling of the type IX secretion system.
Yukitake H, Shoji M, Sato K, Handa Y, Naito M, Imada K, Nakayama K.
Sci Rep.10(1):21109(2020) - Structural basis of strict substrate recognition of l-lysine α-oxidase from Trichoderma viride.
Kondo H, Kitagawa M, Matsumoto Y, Saito M, Amano M, Sugiyama S, Tamura T, Kusakabe H, Inagaki K, Imada K.
Protein Sci.29(11):2213-2225(2020) - PorM, a core component of bacterial type IX secretion system, forms a dimer with a unique kinked-rod shape.
Sato K, Okada K, Nakayama K, Imada K.
Biochem Biophys Res Commun.532(1):114-119(2020) - Structure of polymerized type V pilin reveals assembly mechanism involving protease-mediated strand exchange.
Shibata S, Shoji M, Okada K, Matsunami H, Matthews MM, *Imada K, *Nakayama K, *Wolf M
Nature Microbiology 35(2020) - In situ structure of the Vibrio polar flagellum reveals distinct outer membrane complex and its specific interaction with the stator.
Zhu S, Nishikino T, Takekawa N, Terashima H, Kojima S, Imada K, *Homma M, *Liu J.
J Bacteriol. 202(4):e00592-19(2020) - In Vitro Autonomous Construction of the Flagellar Axial Structure in Inverted Membrane Vesicles.
Terashima H, Tatsumi C, Kawamoto A, Namba K, Minamino T, *Imada K.
Biomolecules. 10(1):E126(2020) - Structure and Energy-Conversion Mechanism of the Bacterial Na+-Driven Flagellar Motor.
Takekawa N, Imada K, Homma M.
Trends Microbiol. 28(9):719-731(2020)
Original Papers
Reviews, Proceedings and Books
2019
- Structural basis of the binding affinity of chemoreceptors Mlp24p and Mlp37p for various amino acids.
#Takahashi Y, #Nishiyama SI, *Kawagishi I, *Imada K.
Biochem Biophys Res Commun. S0006-291X(19)32385-X.(2019) - Second harmonic generation polarization microscopy as a tool for protein structure analysis.
Kaneshiro J, Okada Y, Shima T, Tsujii M, Imada K, *Ichimura T, *Watanabe TM.
Biophys Physicobiol. 16:147-157(2019) - Architecture of the Bacterial Flagellar Distal Rod and Hook of Salmonella.
Saijo-Hamano Y, Matsunami H, Namba K, *Imada K.
Biomolecules. 9(7):E260(2019) - Structural Insights into the Substrate Specificity Switch Mechanism of the Type III Protein Export Apparatus.
Inoue Y, Ogawa Y, Kinoshita M, Terahara N, Shimada M, Kodera N, Ando T, Namba K, *Kitao A, *Imada K, *Minamino T.
Structure. 27(6):965-976(2019) - Structure of the periplasmic domain of SflA involved in spatial regulation of the flagellar biogenesis of Vibrio reveals a TPR/SLR-like fold.
Sakuma M, Nishikawa S, Inaba S, Nishigaki T, Kojima S, Homma M, *Imada K.
J Biochem. 166(2):197-204(2019) - Structure of Vibrio FliL, a New Stomatin-like Protein That Assists the Bacterial Flagellar Motor Function.
Takekawa N, Isumi M, Terashima H, Zhu S, Nishino Y, Sakuma M, Kojima S, Homma M, *Imada K.
MBio. 10(2):e00292-19(2019) - Calcium Ions Modulate Amino Acid Sensing of the Chemoreceptor Mlp24 of Vibrio cholerae.
#Takahashi Y, #Nishiyama SI, Sumita K, Kawagishi I, *Imada K.
J Bacteriol. 201(9):e00779-18(2019)
Original Papers
2018
- In Vitro Reconstitution of Functional Type III Protein Export and Insights into Flagellar Assembly.
Terashima H, Kawamoto A, Tatsumi C, Namba K, Minamino T, *Imada K.
MBio. 9(3):e00988-18(2018) - Insight into structural remodeling of the FlhA ring responsible for bacterial flagellar type III protein export.
#Terahara N, #Inoue Y, Kodera N, Morimoto YV, Uchihashi T, Imada K, Ando T, *Namba K, *Minamino T.
Sci Adv. 4(4):eaao7054(2018) - The helix rearrangement in the periplasmic domain of the flagellar stator B subunit activates peptidoglycan binding and ion Influx.
#Kojima S, #Takao M, #Almira G, Kawahara I, Sakuma M, *Homma M, *Kojima C, *Imada K.
Structure. 26:590-598(2018) - Insight into adaptive remodeling of the rotor ring complex of the bacterial flagellar motor.
Kinoshita M, Furukawa Y, Uchiyama S, Imada K, Namba K, *Minamino T.
Biochem Biophys Res Commun. 496:12-17(2018) - 挿入変異が引き起こすYFPの構造変化.
田中るみか, 慶澤景子, 渡邉朋信, 川口辰也, *今田勝巳.
Spring-8/SACLA利用研究成果集. 6:42-45(2018) - Novel insight into an energy transduction mechanism of the bacterial flagellar type III protein export.
Terashima H, Imada K.
Biophys Physicobiol. 15:173-178(2018) - Bacterial flagellar axial structure and its construction.
*Imada K.
Biophys Rev. 10:559-570(2018)
Original Papers
Reviews, Proceedings and Books
2017
- Assembly and stoichiometry of the core structure of the bacterial flagellar type III export gate complex.
Fukumura T, Makino F, Dietsche T, Kinoshita M, Kato T, Wagner S, *Namba K, *Imada K, *Minamino T.
PLoS Biol. 15:e2002281(2017) - Biochemical characterization of the flagellar stator-associated inner membrane protein FliL from Vibrio alginolyticus.
Kumar A, Isumi M, Sakuma M, Zhu S, Nishino Y, Onoue Y, Kojima S, Miyanoiri Y, Imada K, *Homma M.
J Biochem. 161:331-337(2017) - Structural differences in the bacterial flagellar motor among bacterial species.
Terashima H, Kawamoto A, Morimoto YV, Imada K, *Minamino T.
Biophys Physicobiol. 14:191-198(2017) - 少数で製造を制御する -べん毛をいつどこで、何本生やす？-.
今田勝巳, 小嶋誠司.
実験医学, 35:3218-3223 (2017). - コレラ菌タウリン・アミノ酸走性受容体の同定とそのリガンド認識機構.
西山宗一郎，高橋洋平，今田勝巳，川岸郁朗.
生物物理, 57, 291-295 (2017). -
The Bacterial Flagellum methods and protocols
Chapter 7 Design and preparation of the fragment proteins of the flagellar components suitable for X-ray crystal structure analysis
.
Imada K.
Methods in Molecular Biology (2017) - 少数性生物学
永井健治, 冨樫祐一（編）、
日本評論社4-2 17章 少数で製造をコントロール. p129-138 今田勝巳、2017年3月
Original Papers
Reviews, Proceedings and Books
2016
- Rearrangements of α-helical structures of FlgN chaperone control the binding affinity for its cognate substrates during flagellar type III export.
#Kinoshita M, #Nakanishi Y, Furukawa Y, Namba K, *Imada K, *Minamino T.
Mol. Microbiol. 101: 656-670(2016) - Insight into the flagella type III export revealed by the complex structure of the type III ATPase and its regulator.
*Imada K, Minamino T, Uchida Y, Kinoshita M, Namba K.
Proc. Natl. Acad. Sci. USA. 113: 3633-3638 (2016) - Dependence of fluorescent protein brightness on protein concentration in solution and enhancement of it.
Morikawa TJ, Fujita H, Kitamura A, Horio T, Yamamoto J, Kinjo M, Sasaki A, Machiyama H, Yoshizawa K, Ichimura T, Imada K, Nagai T and *Watanabe TM
Sci. Rep. 6:e22342 (2016) - Identification of a Vibrio cholerae chemoreceptor that senses taurine and amino acids as attractants.
#Nishiyama S, #Takahashi Y, Yamamoto K, Suzuki D, Itoh Y, Sumita K, Uchida Y, Homma M, *Imada K, *Kawagishi I.
Sci. Rep. 6:e20866 (2016). - Epistasis effects of multiple ancestral-consensus amino acid substitutions on the thermal stability of glycerol kinase from Cellulomonas sp. NT3060.
Fukuda Y, Abe A, Tamura T, Kishimoto T, Sogabe A, Akanuma S, Yokobori S, Yamagishi A, *Imada K, *Inagaki K.
J Biosci Bioeng. 121:497-502 (2016).
Original Papers
2015
- Molecular and structural analysis of Legionella DotI gives insights into an inner membrane complex essential for type IV secretion.
#Kuroda T, #Kubori T, Thanh Bui X, Hyakutake A, Uchida Y, *Imada K, *Nagai H.
Sci Rep. 5:e10912 (2015). - Recombinant expression, molecular characterization and crystal structure of antitumor enzyme, l-lysine α-oxidase from Trichoderma viride.
Amano M, Mizuguchi H, Sano T, Kondo H, Shinyashiki K, Inagaki J, Tamura T, Kawaguchi T, Kusakabe H, *Imada K, *Inagaki K.
J Biochem. 157, 549-559 (2015.) - 細菌べん毛モーター固定子の組込みと活性化機構.
今田勝巳, 小嶋誠司.
日本結晶学会誌, 57, 291-296 (2015). - The bacterial flagellar motor and its structural diversity.
Minamino T, Imada K.
Trends Microbiol. 23, 267-274 (2015).
Original Papers
Reviews, Proceedings and Books
2014
- Conformational change in the periplamic region of the flagellar stator coupled with the assembly around the rotor.
#Zhu S, #Takao M, Li N, Sakuma M, Nishino Y, Homma M, Kojima S, *Imada K.
Proc Natl Acad Sci U S A111, 13523-13528 (2014). - Crystallization and preliminary X-ray analysis of the periplasmic domain of FliP, an integral membrane component of the bacterial flagellar type III protein-export apparatus.
Fukumura T, Furukawa Y, Kawaguchi T, Saijo-Hamano Y, Namba K, Imada K, *Minamino T.
Acta Crystallogr F Struct Biol Commun.70(Pt 9), 1215-8 (2014). - べん毛モーターの超高速回転を支える超分子リング構造形成のしくみ
寺島浩行, 本間道夫, 今田勝巳
生物物理, 54, 19-21 (2014).
Original Papers
Reviews, Proceedings and Books
2013
- Interactions of bacterial flagellar chaperone-substrate complexes with FlhA contribute to co-ordinating assembly of the flagellar filament.
Kinoshita M, Hara N, Imada K, Namba K, *Minamino T.
Mol. Micorbiol., 90, 1249-1261 (2013). - Glycine insertion makes yellow fluorescent protein sensitive to hydrostatic pressure.
*Watanabe TM, Imada K, Yoshizawa K, Nishiyama M, Kato C, Abe F, Morikawa TJ, Kinoshita M, Fujita H, Yanagida T.
PLoS One, 8, e73212, (2013). - Common evolutionary origin for the rotor domain of rotary ATPases and Flagellar protein export apparatus.
Kishikawa J, Ibuki T, Nakamura S, Nakanishi A, Minamino T, Miyata T, Namba K, Konno H, Ueno H, *Imada K. and *Yokoyama K.
PLoS One,8, e6469, (2013). - X-ray structure analysis and characterization of AFUEI, an elastase inhibitor from Aspergillus fumigatus.
Sakuma M, Imada K, Okumura Y, Uchiya K, Yamashita N, Ogawa K, Hijikata A, Shirai T, *Homma M, *Nikai T
J Biol Chem., 288, 17451-17459, (2013). - Effects of Chain Length of an Amphipathic Polypeptide Carrying the Repeated Amino Acid Sequence (LETLAKA)n on α-Helix and Fibrous Assembly Formation.
Takei T, Hasegawa K, Imada K, Namba K, Tsumoto K, Kuriki Y, Yoshino M, Yazaki K, Kojima S, Takei T, Ueda T, Miura KI.
Biochemistry, 52, 2810-20, (2013). - Insight into the assembly mechanism in the supramolecular rings of the sodium-driven Vibrio flagellar motor from the structure of FlgT.
Terashima H, Li N, Sakuma M, Koike M, Kojima S, Homma M, *Imada K.
Proc. Natl. Acad. Sci. USA,110: 6133-6138, (2013). - Expression, purification, crystallization and preliminary X-ray diffraction analysis of a core fragment of FlgG, a bacterial flagellar rod protein.
Saijo-Hamano Y, Matsunami H, Namba K, *Imada K.
Acta Crystallogr., F69, 547-550, (2013). - Interaction between FliJ and FlhA, components of the bacterial flagellar type III export apparatus.
Ibuki T, Uchida Y, Hironaka Y, Namba K, *Imada K, Minamino T.
J. Bacteriol., 195, 466-473 (2013).
Original Papers
2012
- Crystallization and preliminary X-ray analysis of the FliH–FliI complex responsible for bacterial flagellar type III protein export.
Uchida Y, Minamino T, Namba K, *Imada K.
Acta Crystallogr., F68, 1311-1314, (2012). - The roles of the dimeric and tetrameric structures of the clock protein KaiB in the generation of circadian oscillations in cyanobacteria.
Murakami R, Mutoh R, Iwase R, Furukawa Y, Imada K, Onai K, Morishita M, Yasui S, Ishii K, Valencia Swain JO, Uzumaki T, Namba K, Ishiura M.
J. Biol. Chem., 287, 29506-29515, (2012). - Phase-dependent generation and transmission of time information by the KaiABC circadian clock oscillator through SasA-KaiC interaction in cyanobacteria.
Valencia SJ, Bitou, K, Ishii K, Murakami R, Morishita M, Onai K, Furukawa Y, Imada K, Namba K, Ishiura M.
Genes to Cells, 17, 398-419, (2012). - Crystallization and preliminary X-ray analysis of FlgA, a Periplasmic protein essential for flagellar P-ring assembly.
Matsunami H, Samatey FA, Nagashima S, Imada K, Namba K.
Acta Crystallogr.,F68, 310-313, (2012). - Interaction of a flagellar chaperone FlgN with FlhA is required for efficient export of its cognate substrates.
Minamino T, Kinoshita M, Hara N, Takeuchi S, Hida A, Koya S, Glenwright H, Imada K, Aldridge PD, Namba K.
Mol. Micorbiol.,83, 775-788 (2012). - Functional defect and restoration of temperature-sensitive mutants of FlhA, a subunit of the flagellar protein export apparatus.
Shimada M, Saijo-Hamano Y, Furukawa Y, Minamino T, *Imada K, Namba K.
J. Mol. Biol., 425, 855-865 (2012). - Interaction between FliI ATPase and a flagellar chaperone FliT during bacterial flagellar protein export.
Minamino T, Kinoshita M, Imada K, Namba K.
Mol. Micorbiol., 83, 168-178 (2012). - The flagellar type III protein export apparatus and F/V type ATPases share a common architecture.
*Imada K, Minamino T, Namba K.
SPring8 Research Frontiers 2011, 24-25 (2012). - べん毛モーターの逆回転のしくみに挑む -熱い論争と3つのモデル-
今田勝巳, 南野徹
化学, 67, 37-41, (2012). - ペリプラズム側構造から見たべん毛モーター構築とモーターの活性化機構
小嶋誠司, 今田勝巳
生物物理, 52, 18-21 (2012). - 輸送シャペロンFliTの構造とべん毛形成制御の分子機構
今田勝巳, 南野徹, 難波啓一
日本結晶学会誌,53, 396-401 (2012).
Original Papers
Reviews, Proceedings and Books
2011
- Ligand specificity determined by differentially arranged common ligand-binding residues in the bacterial amino acid chemoreceptors Tsr and Tar.
Tajima H, *Imada K, Sakuma M, Hattori F, Nara T, Kamo N, Homma M, *Kawagishi I.
J. Biol. Chem.,286, 42200-42210 (2011). - Structural insight into the rotational switching mechanism of the bacterial flagellar motor.
Minamino T, *#Imada K, Kinoshita M, Nakamura S, Morimoto YV, Namba K.
PLoS Biol., 9, e1000616 (2011). - Genetic analysis of the bacteria hook-capping protein FlgD responsible for hook assembly.
Moriya N, Minamino T, Imada K, Namba K.
Microbiology, 157, 1354-1362 (2011). - Common architecture between the flagellar type III protein export apparatus and F- and V-type ATPases.
Ibuki, T, *Imada K, Minamino T, Kato T, Miyata T, Namba K.
Nat. Struct. Mol. Biol., 18, 277-282 (2011).
Original Papers
2010
- The interaction dynamics of a negative feedback loop regulates flagellar number in Salmonella enterica serovar Typhimurium.
Aldridge C, Poonchareon K, Saini S, Ewen T, Soloyva A, Rao CV, Imada K, Minamino T, *Aldridge PD.
Mol. Microbiol. 78, 1416-1430 (2010). - Crystal structure of Legionella DotD: Insights into the relationship between Type IVB and Type II/III secretion systems.
Nakano N, Kubori T, Kinoshita M, *Imada K, *Nagai H.
PLoS Patho., 6, e1001129 (2010). - Structural insight into the regulatory mechanisms of interactions of the flagellar type III chaperone FliT with its binding partners.
*Imada K, Minamino T, Kinoshita M, Furukawa Y, Namba K.
Proc. Natl. Acad. Sci. USA,107, 8812-8817 (2010). - Structure of the cytoplasmic domain of FlhA and implication for flagellar type III protein export.
Saijo-Hamano Y, *#Imada K, Minamino T, Kihara M, Shimada M, Kitao A, Namba K.
Mol. Microbiol., 76, 260-268 (2010). - Role of the C-terminal cytoplasmic domain of FlhA in bacterial flagellar type III protein export.
Minamino T, Shimada M, Okabe M, Saijo-Hamano Y, Imada K, Kihara M, Namba K.
J. Bacteriol., 197, 1929-1936 (2010). - Stator assembly and activation mechanism of the flagellar motor revealed by the crystal structure of the periplasmic region of MotB.
Kojima S, *Imada K, Namba K.
SPring8 Research Frontiers 2009,36-37 (2010). - Insights into the stator assembly of the Na+-driven Vibrio flagellar motor from the crystal structure of MotY.
Kojima S, Homma M, *Imada K.
SPring8 Research Frontiers 2009, 34-35 (2010).
Original Papers
Reviews, Proceedings and Books
2009
- Stator assembly and activation mechanism of the flagellar motor by the periplasmic region of MotB.
Kojima S, *#Imada K, Sakuma M, Sudo Y, Kojima C, Minamino T, Homma M, Namba K.
Mol. Microbiol.,73, 710-718 (2009). - Purification, crystallization and preliminary X-ray analysis of FliT, a bacterial flagellar substrate-specific export chaperone.
Kinoshita M, Yamane M, Matsunami H, Minamino T, Namba K, *Imada K.
Acta Crystallogr., F65, 825-828 (2009). - Crystallization and preliminary X-ray analysis of FliJ, a cytoplasmic component of the flagellar type III protein-export apparatus from Salmonella sp.
Ibuki T, Shimada M, Minamino T, Namba K, *Imada, K.
Acta Crystallogr.,F65, 47-50 (2009). - Crystallization and preliminary X-ray analysis of a C-terminal fragment of FlgJ, a putative flagellar rod cap protein from Salmonella.
Kikuchi Y, Matsunami H, Yamane M, *Imada K, Namba K.
Acta Crystallogr., F65, 17-20 (2009). - Role of the N-terminal domain of FliI ATPase in bacterial flagellar protein export.
Okabe M, *Minamino T, Imada K, Namba K, Kihara M.
FEBS Letters, 583, 743-748 (2009).
Original Papers
2008
- Insights into the stator assembly of the Vibrio flagellar motor from the crystal structure of MotY.
Kojima S, Shinohara A, Terashima H, Yakushi T, Sakuma M, Homma, M, Namba, K, *Imada K.
Proc. Nat’l Acad. Sci. USA,105, 7696-7701 (2008). - Structure and quantum chemical analysis of NAD+-dependent isocitrate dehydrogenase: Hydride transfer and co-factor specificity
*Imada K, Tamura T, Takenaka R, Kobayashi I, Namba K, Inagaki K.
Proteins, 70, 63-71 (2008). - Molecular motors of the bacterial flagella.
Minamino T, Imada K, Namba K.
Curr. Opin. Struc. Biol.18, 1-9 (2008). - Mechanisms of type III protein export for bacterial flagellar assembly.
Minamino T, Imada K, Namba K.
Mol. Biosyst. 4, 1105-1115 (2008). - 細菌べん毛繊維は分子間相互作用とフラストレーションを利用して超らせん構造の変化を制御する
北尾彰朗, 今田勝巳, 難波啓一
生物物理, 48, 11-17 (2008). - 海産性好熱性細菌 Rhodothermus marinus 由来イソアミラーゼの精製，性質検討及びＸ線結晶構造解析
立花亜紀子, 田村隆, 今田勝巳, 木下実紀, 難波啓一, 堤紀子, 橋田みよ子, 坂口博脩, 稲垣賢二
岡山大学農学部学術報告、97, 1-7 (2008). - Extensive structural similarity between the flagellar type III ATPase FliI and F1-ATPase subunits.
Imada K, Minamino T, Namba, K.
SPring8 Research Frontiers 2007, 32-33 (2008). - 最新分子マシン ナノで働く"高度な機械"を目指して
化学同人編集部（編）、
化学同人4-2 タンパク質でできた極小スクリュー 細菌べん毛の分子機構 p104-112 今田勝巳 、2008年4月
Original Papers
Reviews, Proceedings and Books
2007
- PAX6 and SOX2-dependent rgulation of the Sox2 enhancer N-3 involved in embryonic visual system development
Inoue M, Kamachi Y, Matsunami H, Imada K, Uchikawa M, *Kondoh H.
Genes Cells, 12, 1049-1061 (2007). - Gap compression/extension mechanism of bacterial flagellar hook as the molecular universal joint
Furuta T, Samatey FA, Matsunami H, Imada K, Namba K, *Kitao A.
J. Struct. Biol., 157, 481-490 (2007). - Crystallization and preliminary X-ray analysis of MotY, a stator component of the Vibrio Alginolyticus polar flagellar motor
Shinohara A, Sakuma M, Yakushi T, Kojima S, Namba K, Homma M, *Imada K.
Acta Crystallogr., F63, 89-92 (2007). - Structural similarity between the flagellar type III ATPase FliI and F1ATPase subunits.
Imada K, Minamino T, Tahara A, *Namba K.
Proc. Nat’l Acad. Sci. USA,104, 485-490 (2007).
Original Papers
2006
- Coarse-grained molecular dynamics simulations of a rotating bacterial flagellum
Arkhipov A, Freddolino PL, Imada K, Namba K, *Schulten K.
Biophys J.,91, 4589-4597, (2006). - Crystallization and preliminary X-ray analysis of Salmonella FliI, the ATPase component of the type III flagellar protein-export apparatus.
#Minamino T, #*Imada K, #Tahara A, Kihara M, Macnab RM, Namba K.
Acta Crystallogr.,F62, 973-975 (2006). - Switch interactions control energy frustration and multiple flagellar filament structures.
Kitao A, Yonekura K, Maki-Yonekura S, Samatey FA, Imada K, Namba K, *Go N.
Proc. Nat’l Acad. Sci. USA, 103, 4894-4899 (2006).
Original Papers
2005
- Functionally important substructures of circadian clock protein KaiB in a unique tetramer complex
#Iwase R, #Imada K, Hayashi F, Uzumaki T, Morishita M, Onai K, Furukawa Y, Namba K, *Ishiura M.
J. Biol. Chem., 280, 43141-43149 (2005). - Crystallization and preliminary X-ray analysis of C-terminal cytoplasmic domain of FlhA, a membrane protein subunit of the bacterial flagellar type III protein export apparatus.
Saijo-Hamano Y, *Imada K, Minamino T, Kihara M, Macnab RM, Namba K.
Acta Crystallogr., F61, 599-602 (2005). - A partial atomic structure for the flagellar hook of Salmonella typhimurium.
Shailk TR, Thomas DR, Chen JZ, Samatey FA, Matsunami. H, Imada K, Namba K, *DeRosier DJ.
Proc. Nat’l Acad. Sci. USA, 1023-1028 (2005). - べん毛による細菌の泳ぎと方向転換の分子機構
今田勝巳, Fadel A. Samatey, 松波秀行, 長島重広, 北尾彰朗, 米倉功治, 眞木-米倉さおり, 難波啓一
蛋白質 核酸 酵素、50, 1328-1334 (2005). - 生体超分子のユニバーサルジョイント. In silicoで探る細菌べん毛フックのメカニズム
北尾彰朗, Fadel A. Samatey, 松波秀行, 今田勝巳, 難波啓一
蛋白質 核酸 酵素、50, 1335-1340 (2005). - タンパク質科学 構造・物性・機能
後藤祐児、桑島邦博、谷澤克行（編）
化学同人、第３章 超分子アセンブリーとタンパク質ネットワーク２ タンパク質-タンパク質相互作用 p115-130 今田勝巳, 難波啓一、2005年10月
Original Papers
Reviews, Proceedings and Books
2004
- Crystallization and a core fragment of the flagellar hook protein FlgE.
Samatey FA, Matsunami H, Imada K, Nagashima S, *Namba K.
Acta Crystallogr., D60, 2078-2080 (2004). - Structure of the bacterial flagellar hook and implication for the molecular universal joint mechanism.
Samatey FA, Matsunami H, Imada K, Nagashima S, Shailh TR, Thomas DR, Chen JZ, DeRosier DJ, Kitao A, *Namba K.
Nature, 431, 1062-1068 (2004). - Crystallization and preliminary crystallographic analysis of the circadian clock protein KaiB from the thermophilic cyanobacterium Thermosynechococcus elongates BP-1.
Iwase R, *#Imada K, Hayashi F, Uzumaki T, Namba K, *Ishiura M.
Acta Crystallogr.,D60, 727-729 (2004). - 原子構造が明らかにする超分子ナノマシン-細菌べん毛-の動作機構
今田勝巳,
生産と技術、56-4, 46-49 (2004). - ＜１分子＞生物学
合原一幸, 岡田康志, 今田勝巳, 岩田覚, 樺島祥介, 金城政孝, 今野紀雄, 佐甲靖志
岩波書店、2004年9月
Original Papers
Reviews, Proceedings and Books
2003
- ATP-induced hexameric ring structure of the cyanobacterial circadian clock protein KaiC.
Hayashi F, Suzuki H, Iwase R, Uzumaki T, Miyake A, Shen JR, Imada K, Furukawa Y, Yonekura K, Namba K, *Ishiura M.
Genes Cells, 8, 287-96 (2003). - べん毛モーター 超分子機械の構造生物学
今田勝巳, Fadel A. Samatey, 難波啓一
日本結晶学会誌、45, 37-42 (2003).
Original Papers
Reviews, Proceedings and Books
2002
- Biochemical and molecular characterization of the NAD(+)-dependent isocitrate dehydrogenase from the chemolithotroph Acidithiobacillus thiooxidans.
Inoue H, Tamura T, Ehara N, Nishito A, Nakayama Y, Maekawa M, Imada K, Tanaka H, *Inagaki K.
FEMS Microbiol Lett., 214, 127-132 (2002). - Interactions between bacterial flagellar axial proteins in their monomeric state in solution.
Furukawa Y, Imada K, Vonderviszt F, Matsunami H, Sano K, Kutsukake, K, *Namba, K.
J. Mol. Biol., 318, 889-900 (2002). - A Mechanical Switch Found in the Bacterial Flagellar Protofilament Structure.
Samatey FA, Imada K, Namba, K.
SPring8 Research Frontiers 2000/2001,21-22 (2002).
Original Papers
Reviews, Proceedings and Books
2001
- Structure of the bacterial flagellar protofilament and implications for a switch for supercoiling.
Samatey FA, Imada K, Nagashima S, Vonderviszt F, Kumasaka, T, Yamamoto M, *Namba K.
Nature, 410, 331-337 (2001). - 細菌べん毛繊維の自己構築とらせんスイッチ機構
難波啓一, 米倉功治, 眞木さおり, Fadel A. Samatey, 今田勝巳
細胞工学、20, 1371-1379 (2001). - 細菌べん毛素繊維の結晶構造とスイッチ機構
難波啓一, サマティ・ファデル, 今田勝巳, 長島重広
SPring8 Information, 6, 5, 377-382 (2001). - 細菌べん毛の分子構造から自己構築とサブÅ精度のスイッチ機構にせまる
難波啓一, 米倉功治, 眞木さおり, Fadel A. Samatey, 今田勝巳
蛋白質核酸酵素、 46, 1568-1576 (2001).
Original Papers
Reviews, Proceedings and Books
2000
- Crystallization of the F41 fragment of flagellin and data collection from extremely thin crystals.
Samatey FA, Imada K, Vonderviszt F, Shirakibara Y, *Namba K.
J. Struct. Biol., 132, 106-111 (2000). - The bacterial flagellar cap as the rotary promoter of flagellin self-assembly.
Yonekura K, Maki S, Morgan DG, DeRosier DJ, Vonderviszt F, Imada K, *Namba K.
Science,290, 2148-2152 (2000).
Original Papers
1998
- Mechanism of self-association and filament capping by flagellar HAP2.
Vonderviszt F, Imada K, Furukawa Y, Uedaira H, Taniguchi H, *Namba K.
J. Mol. Biol., 284, 1399-1416 (1998). - Structure of 3-isopropylmalate dehydrogenase in complex with 3-isopropylmalate at 2.0Å resolution: the role of Glu88 in the unique substrate-recognition mechanism.
*Imada, K, Inagaki K, Matsunami H, Kawaguchi H, Tanaka H, Tanaka N, Namba K.
Structure, 6, 971-182 (1998). - Assembly characteristics of flagellar cap protein HAP2 of Salmonella: Decamer and pentamer in the pH-sensitive equilibrium.
Imada, K, Vonderviszt F, Furukawa Y, Oosawa K, *Namba K.
J. Mol. Biol., 277, 883-891 (1998). - Plunging interactions of HAP2 pentamer into the distal end of flagellar filament revealed by electron microscopy.
Maki S, Vonderviszt F, Furukawa Y, Imada K, *Namba K.
J. Mol. Biol., 277, 771-777 (1998). - 生化学辞典 第3版 東京化学同人
今堀和友, 山川民夫 監修
東京化学同人 1998年10月
Original Papers
Reviews, Proceedings and Books
1993
- 高度好熱菌の３-イソプロピルリンゴ酸脱水素酵素の構造-酵素研究の新しいモデル分子-
今田勝巳, 山岸明彦, 大島泰郎
蛋白質･核酸･酵素, 38, 1453-1465 (1993). - 高度好熱菌由来３-イソプロピルリンゴ酸脱水素酵素（IPMDH）のX線結晶構造解析
今田勝巳
蛋白質工学基礎研究センターだより、 14, 14-24 (1993).
Reviews, Proceedings and Books
1992
- Crystallization and preliminary X-ray studies of a Bacillus-subitilus and Thermus-thermophilus HB8 chimeric 3-isopropylmalate dehydrogenase and thermostable mutants of it.
Sakurai M, Onodera K, Moriyama H, Matsumoto O, Tanaka N, Numata K, Imada K, Sato M, Katsube Y, Oshima T.
J.Biochem., 112, 173-174 (1992). - A high-speed data-collection system for large-unit-cell crystals using an imaging plate as a detector.
*Sato M, Yamamoto M, Imada K, Katube Y.
J. Appl. Cryst., 25, 348-357 (1992).
Original Papers
1991
- Three-dimensional structure of a highly thermostable enzyme, 3-isopropylmalate dehydrogenase of Thermus thermophilus at 2.2Å resolution.
Imada K, *Sato M, Tanaka N, Katusbe Y, Matsuura Y, Oshima T.
J. Mol. Biol., 222, 725-738 (1991). - Crystallization and preliminary X-ray studies of a Bacillus-subitilus and Thermus-thermophilus HB8 chimeric 3-isopropylmalate dehydrogenase.
Onodera K, Moriyama H, Takenaka A, Tanaka N, Akutsu N, Muro M, Oshima T, Imada K, Sato M, Katsube Y.
J.Biochem., 109, 1-2 (1991).
Original Papers
博士学位論文
2018年度
| 高橋 洋平 | Lipid recognition mechanism of dCACHE type chemoreceptors of Vibrio cholerae （コレラ菌dCACHE型走化性受容体のリガンド認識機構） |
|---|
修士論文
2024年度
| 青山 未幸 | コレラ菌走化性受容体SatA-Mlp3システムの分子機構 |
|---|---|
| 安藤 優介 | Porphyromonas gingivalisの線毛蛋白質FimDの構造解析 |
| 阪田 惇史 | べん毛蛋白質Flkの性質とL-リシン酸化酵素の結晶構造解析 |
| Lee Changwoo | 細菌べん毛輸送アッセイ系の改良とべん毛タンパク質の輸送解析 |
2023年度
| 上田 悠加 | 放線菌由来L-グルタミン酸酸化酵素の基質認識および活性発現機構 |
|---|---|
| 碓井亜瑳子 | べん毛輸送ATPase FliI 6量体の構造変化 |
| 橋本 紗依 | クライオ電子顕微鏡による細菌べん毛フック成長端の構造解析 |
2022年度
| 五十嵐玲香 | 細菌べん毛Class2蛋白質の輸送解析 |
|---|---|
| 上堀まりあ | 細菌べん毛モーター固定子の構造変化の解析と固定子の回転計測実験系の構築 |
| 大坪 史歩 | 新規赤色蛍光蛋白質のX線結晶構造解析による、赤色発色団の形成機構の解明 |
| 森 来未 | べん毛成長末端の構造解析と新しいべん毛精製法の確立 |
2021年度
| 小島 嶺 | Porphyromonas gingivalisの線毛蛋白質の構造解析 |
|---|---|
| 坂岡 伸哉 | 細菌べん毛Class3蛋白質の輸送解析 |
| 矢野 達就 | べん毛輸送ATPase複合体の構造変化と作動機構の研究 |
| 藪中 柚輝 | コレラ菌走化性受容体Mlp3,Mlp8のリガンド認識機構 |
| 山下 俊貴 | 細菌べん毛モーター固定子の回転計測を目指した実験系の構築 |
2020年度
| 池田 篤志 | フィラメント形成直前のべん毛先端の構造解析 |
|---|---|
| 酒井 源志 | 輸送切り替え機構の解明を目指した細菌べん毛蛋白質FlhA変異体の構造解析 |
| 松田 峻汰 | 変異によるL-リシン酸化酵素の基質特異性変換の構造基盤 |
| 山口 綾香 | ナトリウム駆動形べん毛固定子の固定機構 |
2019年度
| 岩井 朋也 | レジオネラ菌エフェクター蛋白質LotAのX線結晶構造解析 |
|---|---|
| 辻本 拓海 | べん毛フック成長端の構造解析 |
| 東権 幸樹 | べん毛蛋白質輸送に基質相互が及ぼす影響の解析 |
| 水口あかり | 細菌べん毛蛋白質輸送の基質特異性解析および定量解析への試み |
| 渡辺 翔子 | Type I-D CRISPR-CasシステムのエンドリボヌクレアーゼCas6の構造解析 |
2018年度
| 岡田 広大 | Porphyromonas gingivitisの線毛蛋白質FimAの構造 |
|---|---|
| 北川 征樹 | 高基質特異性L−リシン酸化酵素の活性化機構及び基質認識機構の解明 |
| 壽山 翔太 | べん毛輸送ゲート蛋白質FlhA膜領域の集合体形成 |
| 高田 啓矢 | 反転膜小胞を用いたべん毛軸構造構成蛋白質の輸送順序解析 |
| 都築 侑果 | コレラ菌走化性受容体Mlp8, Mlp37の構造解析 |
| 西内 光希 | Bacillus属菌べん毛モーター固定子蛋白質MotSの構造解析 |
2017年度
| 木田 葵 | べん毛フィラメントキャップの構造 |
|---|---|
| 辻井 美香 | 生体内圧力センサーの創成を目指した、蛍光タンパク質の圧力応答機構の解明 |
| 戸野 侃 | 反転膜を用いたべん毛III型蛋白質輸送の蛍光計測 |
2016年度
| 伊角 実優 | 固定子に作用するべん毛内膜蛋白質FliLの構造解析 |
|---|---|
| 伊藤菜奈子 | 高基質特異性L-アミノ酸酸化酵素の基質特異性発現機構と活性発現機構の解明 |
| 反田 祐介 | IX型分泌装置分泌タンパク質PGN0123の構造解析と機能予測 |
| 松本 雄大 | 反転膜小胞を用いたべん毛軸構造蛋白質の輸送順序の解析 |
2015年度
| 高橋 洋平 | コレラ菌走化性受容体のリガンド認識機構 |
|---|---|
| 佐藤 充眞 | シンジオタクチックポリスチレン(sPS)と鎖状分子および高分子化合物との共晶化 |
2014年度
| 小川 雄也 | べん毛３型輸送装置蛋白質FlhAの細胞質領域の構造変化 |
|---|---|
| 近藤 裕輝 | 高基質特異性L-リシン酸化酵素の基質認識機構の解明 |
| 田中るみか | 黄色蛍光タンパク質挿入変異体の系統的な構造解析 |
| 松島 和司 | べん毛C-ringを構成するFliM-FliN複合体のストイキオメトリとべん毛輸送 |
2013年度
| 黒田 琢弥 | IVB型分泌系コア複合体蛋白質DotI/TraMの構造 |
|---|---|
| 瀬戸 直樹 | シンジオタクチックポリスチレン共結晶のゲスト交換機構に関する研究 |
| 中西 雄紀 | 多機能性を持つ細菌べん毛蛋白質シャペロンFlgNのX線結晶構造解析 |
2012年度
| 佐野 宰久 | 高基質特異性L-アミノ酸酸化酵素の基質認識の構造基盤 |
|---|
2011年度
| 佐々木啓太 | シンジオタクチックポリスチレンと種々のゲストとの結晶性抱接錯体形成 |
|---|---|
| 高尾 真登 | 細菌べん毛モーター固定子タンパク質のペリプラズム領域の結晶構造 |
| 竹村 和也 | 疎水性基をもつアミノ酸に置換されたコラーゲンモデルペプチドの構造研究 |
プレスリリース
| 2025/01/17 | 「ナトリウムを見分けて通す」 細菌のべん毛モーター固定子の構造を解明 |
|---|---|
| 2023/10/24 | 緑色蛍光蛋白質を赤色に |
| 2021/07/09 | 細菌の回転モーターの複雑な回転子構造を解明 |
| 2021/03/05 | 細菌べん毛モーターの基礎となるMSリングの構造を解明 |
| 2020/04/14 | 歯周病をもたらす歯周病菌5型線毛の構造と線毛形成のしくみを解明 |
| 2019/03/20 | 細菌べん毛モーターの出力強化蛋白質FliLのしくみを解明 |
| 2018/06/28 | 細菌のIII型分泌装置の機能を試験管中で再現！ |
| 2018/04/26 | 細菌べん毛タンパク質輸送の交通整理のしくみを解明 |
| 2018/03/23 | 細菌べん毛モーターが回る鍵、固定子を“固定”するしくみを解明！ |
| 2017/08/04 | 細菌べん毛III型輸送装置ができるしくみを解明 |
| 2016/03/15 | 細菌の分泌装置蛋白質の構造を原子レベルで解明！ |
| 2016/03/09 | 分子混雑が計測できる蛍光タンパク質「GimRET」の開発 |
| 2016/02/17 | コレラ菌が胆汁を目指して泳ぐしくみを解明！ |
| 2014/09/03 | 細菌べん毛モーターは伸び縮みにより活性化 |